Blind deconvolution in fluorescence microscopy

A good estimate of the PSF is mandatory to perform non blind deconvolution. In wide field fluorescence microscopy, such an estimate is derived of theoretical models or calibrated using fluorescent beads. However, using only known parameters about the setup (wavelength, NA, refractive index,...), theoretical models lack flexibility to represents the wide variety of PSFs. Similarly, measured PSFs are often noisy due to the small size of calibration beads, especially at high numerical aperture.

coupesResultat en

Blind deconvolution algorithms bypass the problem of PSF calibration

Blind deconvolution algorithms bypass the problem of PSF calibration by simultaneously estimating the PSF and the object. The 3D PSF is modeled after a parametrized pupil function. The PSF parameters are estimated jointly with the object in a maximum a posteriori framework.

Results

In wide field fluorescence microscopy, our method outperforms state-of-the art blind and non-blind deconvolution methods and gives a depth resolution close to the lateral resolution.


A «learn 2D, apply 3D» method for 3D deconvolution microscopy by Ferréol Soulez in 11th IEEE International Symposium on Biomedical Imaging, Beijing (China, 2014).
https://hal.archives-ouvertes.fr/hal-00914839

Blind deconvolution of 3D data in wide field fluorescence microscopy
by F. Soulez, L. Denis, Y. Tourneur and E. Thiébaut in 9th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, Barcelona, Spain, May 2012. (pdf, slides).